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ls columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec ls columns
    Ls Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 8965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/column/LS+Columns/custom%40130-042-401%4042831169
    Average 99 stars, based on 8965 article reviews
    ls columns - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Single Cell:

    Article Title: Single-Nuclei Transcriptomic Characterization of APOE4 -Associated Alzheimer’s Disease
    Article Snippet: For single-nuclei RNA sequencing (snRNA-seq), fresh frozen tissue was sent to Azenta Life Sciences for sample preparation. .. To prepare single cell suspensions, myelin debris was removed (Myelin Removal Beads II; Miltenyi Biotec; 130-096-433) and samples were passed through a column (LS Columns; Miltenyi Biotec; 130-042-401). .. Samples were dissociated using the gentleMACS Program (Miltenyi Biotec) in Nuclei Extraction Buffer (Miltenyi Biotec; 130-128-024) with RNase inhibitor to generate single nuclei suspensions according to the manufacturer’s protocol.

    Article Title: Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts.
    Article Snippet: .. Then, tissue was digested through the commercial kit Neonatal Heart Dissociation Kit, mouse and rat (Miltenyi Biotec; #130-098-373), obtaining a single cell suspension, and cardiomyocytes were negatively selected through an antibody-based chromatographic column (Miltenyi Biotec; # 130105-420) following the manufacturer instructions. .. Plates were coated with 1% gelatin (SigmaAldrich; G1890), and cardiomyocytes were diluted 10/ml in custom-made ‘seeding medium’ (10% horse serum, 100μM BrdU).

    Article Title: Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts.
    Article Snippet: .. Then, tissue was digested through the commercial kit Neonatal Heart Dissociation Kit, mouse and rat (Miltenyi Biotec; #130-098-373), obtaining a single cell suspension, and cardiomyocytes were negatively selected through an antibody-based chromatographic column (Miltenyi Biotec; # 130- 105-420) following the manufacturer instructions. .. Plates were coated with 1% gelatin (SigmaAldrich; G1890), and cardiomyocytes were diluted 106/ml in custom-made ‘seeding medium’ (10% horse serum, 100μM BrdU).

    Magnetic Cell Separation:

    Article Title: Single-cell analysis of Plasmodium falciparum transcripts after drug perturbation identifies feedback regulation as well as increased transmission potential
    Article Snippet: .. After 48 hours of drug exposure, the culture was passed through a column using magnetic cell separation (MACS, Miltenyi Biotec #130-042-901), where infected red blood cells were retained in the column and washed with non-supplemented RPMI medium (except for replicate 1). ..

    Article Title: WDFY4-dependent cross-presentation proceeds via a vacuolar antigen-processing route.
    Article Snippet: .. Dendritic cells were then enriched by positive selection using CD11c microbeads and MACS LS column (Miltenyi Biotec, 130- 125- 835) Antigen Preparation. ..

    Infection:

    Article Title: Single-cell analysis of Plasmodium falciparum transcripts after drug perturbation identifies feedback regulation as well as increased transmission potential
    Article Snippet: .. After 48 hours of drug exposure, the culture was passed through a column using magnetic cell separation (MACS, Miltenyi Biotec #130-042-901), where infected red blood cells were retained in the column and washed with non-supplemented RPMI medium (except for replicate 1). ..

    Selection:

    Article Title: WDFY4-dependent cross-presentation proceeds via a vacuolar antigen-processing route.
    Article Snippet: .. Dendritic cells were then enriched by positive selection using CD11c microbeads and MACS LS column (Miltenyi Biotec, 130- 125- 835) Antigen Preparation. ..

    Tissue:

    Article Title: Context-dependent CCL2-CCR2 signaling drives distinct immune infiltration programs in traumatic and autoimmune CNS disorders.
    Article Snippet: After filtration, take exactly 5 mL for washing, centrifuge at 300 g for 5 min at 4 ◦C, and then aspirate the supernatant (Cat# 130–092-628, Miltenyi). (3) Myelin Sheath Removal: Add a mixture of 180 μL 5% BSA-PBS and 20 μL Beads to each tube, incubate at 4 ◦C for 20 min, then add 2 mL of 5% BSA-PBS, centrifuge at 300 g for 5 min at 4 ◦C, and resuspend the pellet in 500 μL of 5% BSA-PBS. .. Employ the magnetic bead method to transfer the tissue fluid into an MS Column, ensuring to rinse the column before adding the sample (Cat# 130–096- 433, Miltenyi). (4) Blocking: Centrifuge the filtered sample, which has a total volume of 2 mL, and resuspend it in 210 μL of 5% BSA-PBS. ..

    Mass Spectrometry:

    Article Title: Context-dependent CCL2-CCR2 signaling drives distinct immune infiltration programs in traumatic and autoimmune CNS disorders.
    Article Snippet: After filtration, take exactly 5 mL for washing, centrifuge at 300 g for 5 min at 4 ◦C, and then aspirate the supernatant (Cat# 130–092-628, Miltenyi). (3) Myelin Sheath Removal: Add a mixture of 180 μL 5% BSA-PBS and 20 μL Beads to each tube, incubate at 4 ◦C for 20 min, then add 2 mL of 5% BSA-PBS, centrifuge at 300 g for 5 min at 4 ◦C, and resuspend the pellet in 500 μL of 5% BSA-PBS. .. Employ the magnetic bead method to transfer the tissue fluid into an MS Column, ensuring to rinse the column before adding the sample (Cat# 130–096- 433, Miltenyi). (4) Blocking: Centrifuge the filtered sample, which has a total volume of 2 mL, and resuspend it in 210 μL of 5% BSA-PBS. ..

    Blocking Assay:

    Article Title: Context-dependent CCL2-CCR2 signaling drives distinct immune infiltration programs in traumatic and autoimmune CNS disorders.
    Article Snippet: After filtration, take exactly 5 mL for washing, centrifuge at 300 g for 5 min at 4 ◦C, and then aspirate the supernatant (Cat# 130–092-628, Miltenyi). (3) Myelin Sheath Removal: Add a mixture of 180 μL 5% BSA-PBS and 20 μL Beads to each tube, incubate at 4 ◦C for 20 min, then add 2 mL of 5% BSA-PBS, centrifuge at 300 g for 5 min at 4 ◦C, and resuspend the pellet in 500 μL of 5% BSA-PBS. .. Employ the magnetic bead method to transfer the tissue fluid into an MS Column, ensuring to rinse the column before adding the sample (Cat# 130–096- 433, Miltenyi). (4) Blocking: Centrifuge the filtered sample, which has a total volume of 2 mL, and resuspend it in 210 μL of 5% BSA-PBS. ..

    Suspension:

    Article Title: Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts.
    Article Snippet: .. Then, tissue was digested through the commercial kit Neonatal Heart Dissociation Kit, mouse and rat (Miltenyi Biotec; #130-098-373), obtaining a single cell suspension, and cardiomyocytes were negatively selected through an antibody-based chromatographic column (Miltenyi Biotec; # 130105-420) following the manufacturer instructions. .. Plates were coated with 1% gelatin (SigmaAldrich; G1890), and cardiomyocytes were diluted 10/ml in custom-made ‘seeding medium’ (10% horse serum, 100μM BrdU).

    Article Title: Effect of the cardiac long non-coding RNA Charme depletion on the maturation and paracrine signaling of resident cardiac fibroblasts.
    Article Snippet: .. Then, tissue was digested through the commercial kit Neonatal Heart Dissociation Kit, mouse and rat (Miltenyi Biotec; #130-098-373), obtaining a single cell suspension, and cardiomyocytes were negatively selected through an antibody-based chromatographic column (Miltenyi Biotec; # 130- 105-420) following the manufacturer instructions. .. Plates were coated with 1% gelatin (SigmaAldrich; G1890), and cardiomyocytes were diluted 106/ml in custom-made ‘seeding medium’ (10% horse serum, 100μM BrdU).



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